Figure 6.

T-cell adhesion to HA is resistant to shear stress flow conditions. Purified human T cells were treated with RANTES or MIP-1β (each at 40 ng/ml), TNF-α (20 ng/ml) or IL-2 (50 U/ml), seeded onto HA-coated surfaces, and exposed to shear stress (2 min). The shear stresses tested were 150 s⁻¹, 1500 s⁻¹, or none (static conditions). The cells that still remained attached after these treatments were visualized and counted. Cell adhesion to HA in the absence of activation and shear flow was considered as control. These results are the average (and SD) of four experiments. ▪, static conditions; ○, 150 s⁻¹; ▵, 1500 s⁻¹.