Semiquantitative RT-PCR for IFN-γ (a), IL-4 (b), IL-1 (c) and TNF-α (d) in the lungs of BALB/c mice infected with M. tuberculosis H37Rv, and treated with the indicated regimen from day 60. There were not sufficient duplicates for error bars or statistics. However, the findings help to confirm the ELISA and immunohistochemical data. (□) control infected mice; (○) mice treated day 60 and day 90 with M. vaccae 1·0 mg; (▪) mice treated from day 60, three times per week with AED + corticosterone; (•) AED + corticosterone and 1·0 mg M. vaccae.