Figure 4.

RT-PCR analysis of vaginal T-cell surface marker expression. (a) RT-PCR of total RNA extracted from vaginal lymphoid cells (VL) (5 × 10⁶), whole vaginal tissue (VAG), and lymph node tissue (LN) of CBA/J mice was performed using Taq DNA polymerase and primers designed to amplify CD3 (549 bp), CD8 (513 bp), CD4 (615 bp), CD4B 983 bp, TCR-β (268 bp) and TCR-δ (222 bp) chain constant regions of murine systemic T lymphocytes. Amplification with primers for GAPDH (239 bp) was used as an internal control. The figure is representative of at least five experiments using different mice. (b) Southern blot analysis of CD4 and CD4B amplification products derived from vaginal and lymph node tissue cDNA using a γ-³²P-end-labelled probe internal of the predicted amplification product.