Figure 1.

Time-course of the effect of calcitonin gene-related peptide (CGRP) on lipopolysaccharide (LPS)-induced interleukin-12 (IL-12) production in mouse peritoneal macrophages (Mφ). Mφ were stimulated with LPS (0·1 µg/ml), in the presence or absence of 100 nm CGRP, and the supernatants were harvested at different time-points. IL-12 released into the medium was assayed by using an enzyme-linked immunosorbent assay (ELISA). The inhibitory effect of CGRP on LPS-induced IL-12 release occurred as early as 6 hr after addition of CGRP, with a maximal effect at 24 hr. The data shown represent the mean ± SEM. *P < 0·05, compared with LPS alone at same time-point; n = 4 for each group.