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. 2000 Sep;101(1):61–67. doi: 10.1046/j.1365-2567.2000.00082.x

Figure 6.

Figure 6

Reverse transcription–polymerase chain reaction (RT–PCR) analysis of the calcitonin gene-related peptide (CGRP) effect on interleukin-12 (IL-12) mRNA expression. Macrophages (Mφ) were stimulated with 0·1 µg/ml of lipopolysaccharide (LPS) in the presence or absence of 100 nm CGRP and/or 1 µm H89 for 5 hr. The mRNA level of IL-12 p40 was reduced to a greater extent by CGRP in the absence of 1 µm H89 (a). However, the IL-12 p35 mRNA was not affected by CGRP on stimulated Mφ (c). Bands were quantified densitometrically and are expressed as mRNA ratio of IL-12 p40 to β-actin (internal standard) in the same experiment (b). This result was representative of three similar, independent experiments.