Figure 7.

IFN-γ production and proliferative response of the PEC DN TCRαβ⁺ T cells in C57BL/6 mice. Mice were infected with 1 × 10⁵ PFU intraperitoneally and PEC were aseptically harvested on day 5 after infection. The CD4⁺ and CD8⁺ T cells from plastic non-adherent cells were magnetically depleted and 1 × 10⁵ viable cells were cultured in 0·2 ml RPMI in 96-well flat-bottomed tissue culture plates coated 24 hr before with purified anti-TCRβ mAb (H57-597). After 3 days culture at 37° 0·1 ml supernatant from each well was collected and IFN-γ was measured by ELISA. The remaining cultured cells were pulsed with 1 µCi/well [³H]TdR and cultured for another 6 hr at 37°. The cells were then harvested and thymidine incorporation was determined by liquid scintillation counter. The figure represents one of two independent experiments. *P < 0·05 (Student's t-test).