Table 1.
Modulation of DN TCRαβ+ T-cell surface markers in naı¨ve and MCMV-infected C57BL/6 mice
| % Surface markers | |||||
|---|---|---|---|---|---|
| Organ | Days after infection | CD69 | CD25 | CD44 | CD62L |
| PEC | Day 0 | 2·41 ± 1·1 | 0·54 ± 0·9 | 85·00 ± 7·7 | 24·45 ± 2·7 |
| Day 5 | 77·17 ± 7·6** | 14·30 ± 3·9** | 75·46 ± 14·3 | 42·81 ± 6·7* | |
| Liver | Day 0 | 8·28 ± 2·0 | 0·14 ± 0·1 | 89·36 ± 4·7 | 2·95 ± 0·4 |
| Day 5 | 51·58 ± 5·1** | 2·75 ± 0·1** | 88·41 ± 0·8 | 7·04 ± 1·9* | |
| Spleen | Day 0 | 7·74 ± 0·5 | 7·30 ± 3·3 | 43·47 ± 4·6 | 6·99 ± 2·7 |
| Day 5 | 12·12 ± 2·4* | 66·21 ± 4·5** | 21·69 ± 1·8* | 0·11 ± 0·2* | |
C57BL/6 mice were infected intraperitoneally with 1 × 105 PFU. PEC, liver and spleen cells were collected and percentages of CD69-, CD25-, CD44- and CD62L-positive DN TCRαβ+ T cells were determined by FACS analysis as described in the Materials and Methods.
*
P < 0·05
**
P < 0·005 (Student's t-test).