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The treatment with NiCl2 and DNCB induced several phenotypic changes on TGF-β1+ DCs. TGF-β1+ DCs differentiated from peripheral blood CD14+ monocytes by GM-CSF, IL-4 and TGF-β1 were treated with 300 µm of NiCl2 or 30 µm of DNCB for 48 hr. These hapten-treated TGF-β1+ DCs were analysed by flow cytometry for their expression of the molecules related to the function of LCs. These are representative data from five different experiments that showed similar results.
