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. 2000 Sep;101(1):112–119. doi: 10.1046/j.1365-2567.2000.00078.x

Figure 2.

Figure 2

Sera from severe atopic individuals contain immunoglobulin E (IgE) autoantibodies. Nitrocellulose-blotted proteins from a human epithelial cell line (A431) (a) and from human platelets (b) were exposed to sera from atopic patients suffering from symptoms of atopy (lanes A–J) (see Tables 1 and 2) and, for control purposes, with serum from a non-atopic individual (lane K) or with buffer alone (lane L). Bound IgE was detected with 125I-labelled anti-human IgE antibodies. Molecular weights (× 10−3) are displayed on the left of the figure.