Figure 2.
C1q-opsonized L. monocytogenes and adsorbed C1q onto the wells induce O2– production and correlates with the bacterial ingestion. (a) O2– production was measured in IC-21 cells (at 3 × 105/well) incubated with the following stimuli: C1q (100 µg/ml) adsorbed to plates or added in solution. (b) In this experiment IC-21 cells (at 3 × 105/well) were exposed to 1 × 105 or 1 × 106L. monocytogenes previously opsonized or not with C1q (100 µg/ml). This shows O2– production measured as described after 60 min incubation with each stimulus. (c) A kinetic study of O2– production by IFN-γ-treated IC-21 cells infected with 1 × 105L. monocytogenes opsonized with or without C1q. Data are presented as the mean ± SD nmol of O2– produced per mg of protein in three different experiments.