Figure 4.
C1q potentiates the NO2– production induced by different stimuli. (a) NO2– production is shown for IFN-γ-treated IC-21 cells (at 3 × 105/well) incubated for 48 hr (with or without IFN-γ[100 U/ml]) in plates with (5 µg/ml) or without C1q adsorbed on wells in the presence or absence of bacteria (1 × 105). (b) Comparison of the NO2– response of IC-21 cells to varying concentrations of C1q used to opsonize L. monocytogenes (1 × 105 bacteria) in the presence or not of IFN-γ (100 U/ml). Results are expressed as the mean ± SD of eight determinations of nmol of NO2– produced/well.