Figure 6.
IC-21 cells kill intracellular C1q-opsonized L. monocytogenes by an oxygen and nitrogen-dependent mechanism. IC-21 cells were incubated for 3 hr with or without the inhibitors indicated and then, infected with bacteria previously opsonized with (100 µg/ml) or without C1q for 60 min at 4°. Gentamicin (5 µg/ml) was added after 1 hr. Values are expressed as replication index of L. monocytogenes at different times. Data represent the mean replication index of three experiments. *P < 0·05; **P < 0·01.