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. 2000 Nov;101(3):378–387. doi: 10.1046/j.1365-2567.2000.00124.x

Figure 1.

Figure 1

Reverse transcription–polymerase chain reaction (RT–PCR) specific for porcine cytokines and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Total RNA was isolated from concanavalin A (Con A)-stimulated (24 hr) peripheral blood mononuclear cells (PBMC) of a naive pig and used for RT–PCR specific for: GAPDH (lane 1), interleukin (IL)-2 (lane 2), interferon-γ (IFN-γ) (lane 3), IL-4 (lane 4) and IL-10 (lane 5). The PCR products were separated in a 2% agarose gel; the sizes (in bp) for each specific product are indicated. M, molecular-weight markers.