Figure 4.

Kinetics of amplification of glyceraldehyde-3-phosphate dehydrogenase (GAPDH)-specific target cDNA and MIMIC. Identical amounts of both templates (10 amol) were co-amplified, parts of the reaction separated in a 2% agarose gel at the indicated number of polymerase chain reaction (PCR) cycles, and the amount of each PCR product determined densitometrically. The slope of both curves indicated very similar efficiency of amplification for the target cDNA (filled circles) and the MIMIC (open circles).