Table 1.
Primers used for quantitative, competitive reverse transcription–polymerase chain reaction (RT–qcPCR)
| Product size (bp) | ||||
|---|---|---|---|---|
| Target | Primer sequence (5′−3′) | Target | MIMIC | GenBank acc. no. |
| IL-2 | Forward GCACCTACTTCAAGCTCTAC | 387 | 431 | X56750 |
| Reverse GATGCTTTGACAAAAGGTAATC | ||||
| IL-4 | Forward TATTCATGGGTCTCACCTCCCA | 337 | 432 | X68330 |
| Reverse TTGGCTTCATGCACAGAACAG | ||||
| IL-10 | Forward TACCTGGGTTGCCAAGCCTT | 523 | 431 | L20001 |
| Reverse TTCACAGAGAGGCTCGGTAAAT | ||||
| IFN-γ | Forward ATTTTGAAGAATTGGAAAGAGG | 368 | 433 | X53085 |
| Reverse AAATTCAAATATTGCAGGCAGG | ||||
| GAPDH | Forward CCTTCATTGACCTCAACTACAT | 400 | 432 | U48832 |
| Reverse CCAAAGTTGTCATGGATGACC | ||||
GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IFN-γ, interferon-γ; IL, interleukin.