Figure 1.

Phenotype and cytokine profiles of adherent and floating DC. DC from rat bone marrow were cultured for 7 days in the presence of GM-CSF and IL-4. Adherent DC and floating DC were isolated. (a) Adherent DC and floating DC were analysed by flow cytometry for CD54 surface marker. (b) DC were stained with PE-conjugated anti-rat IL-10 and then examined by flow cytometry. (c) DC were examined for IL-10 and IL-12 mRNA expression by in situ hybridization as described in the Materials and methods. Data are representative of three independent experiments; *P < 0·05; **P < 0·01; ***P < 0·001.