Table 1.

Comparison of immunohistological characteristics at different times after SD-rat islet transplantation between normal and sensitized mice

	Day 1		Day 2		Day 3		Day 4		Rejection
Parameters	c n=3	s n=3	c n=3	s n=3	c n=3	s n=3	c n=3	s n=3	c n=3	s n=3
IgM	+	+	+	+	+	+	+	+	+	+
IgG	±	++	±	++	±	++	−	+	−	+
IgA	±	++	±	++	±	++	+	++	±	++
C3	++	++	++	++	+	+	±	+	−	±
CD4	−	−	−	+	−	++	+	++	++	++
CD8	−	−	−	+	−	+	+	++	++	++
Mac-1	±	++	±	++	±	++	+	++	++	++

Nephrectomy was performed at days 1, 2, 3, 4 after islet transplantation and at rejection in control (c) and sensitized (s) mice. Serial frozen kidney sections were cut at 4 µm intervals and stained with anti-IgM, IgG, IgA, C3, CD4, CD8, Mac-1 antibody and corresponding fluoroscein isothiocyanate-conjugated second antibody as described in Materials and methods. The results were expressed as negative (−), weak (±), positive (+) and strongly positive (++) depending on the fluorescence intensity.