Table 1.
Day 1 | Day 2 | Day 3 | Day 4 | Rejection | ||||||
---|---|---|---|---|---|---|---|---|---|---|
Parameters | c n=3 | s n=3 | c n=3 | s n=3 | c n=3 | s n=3 | c n=3 | s n=3 | c n=3 | s n=3 |
IgM | + | + | + | + | + | + | + | + | + | + |
IgG | ± | ++ | ± | ++ | ± | ++ | − | + | − | + |
IgA | ± | ++ | ± | ++ | ± | ++ | + | ++ | ± | ++ |
C3 | ++ | ++ | ++ | ++ | + | + | ± | + | − | ± |
CD4 | − | − | − | + | − | ++ | + | ++ | ++ | ++ |
CD8 | − | − | − | + | − | + | + | ++ | ++ | ++ |
Mac-1 | ± | ++ | ± | ++ | ± | ++ | + | ++ | ++ | ++ |
Nephrectomy was performed at days 1, 2, 3, 4 after islet transplantation and at rejection in control (c) and sensitized (s) mice. Serial frozen kidney sections were cut at 4 µm intervals and stained with anti-IgM, IgG, IgA, C3, CD4, CD8, Mac-1 antibody and corresponding fluoroscein isothiocyanate-conjugated second antibody as described in Materials and methods. The results were expressed as negative (−), weak (±), positive (+) and strongly positive (++) depending on the fluorescence intensity.