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Immunoblot analysis of recombinant proteins. Serial dilutions of rsCD59‐Crry, rsCrry and rsCD59 were applied to nitrocellulose and detected with monoclonal antibody 6D1 anti‐rat CD59 or polyclonal anti‐rat Crry. These results confirm the reactivity of anti‐Crry and anti‐CD59 with the corresponding recombinant protein and that the chimeric rsCD59‐Crry retains reactivity with both antibodies.
