Figure 5.

DNA vaccination fails to induce protection against infection of a peripheral organ. C57BL/6 mice were immunized with pEGFPL33A or pEGFPL33A/ISS as described for Fig. 2. Ten days after immunization mice were challenged i.p. with 2 × 10⁶ PFU recombinant vaccinia virus expressing LCMV GP (Vacc-G2) or with irrelevant recombinant vaccinia virus (Vacc-VSV) as controls. LCMV-infected mice served as controls for virus clearance. Four days after the vaccinia challenge infection, vaccinia titres were measured in the ovaries.