Figure 1.

ACAID induction when PEC donors are C57BL/6, B6.lpr/lpr and B6.gld/gld mice. PEC from C57BL/6, B6.lpr/lpr and B6.gld/gld mice were incubated overnight (treatment) in the presence of TGF-β2 and OVA (5 mg/ml). The next day, the cells were washed three times with HBSS medium and injected (2 × 10⁴) i.v. into syngeneic recipients. Seven days later these recipients were immunized with OVA (100 µg) in CFA s.c. and 1 week later their ears were challenged with OVA (400 µg). Naive mice were only ear challenged with OVA. Ear swelling responses after 24 hr are presented as mean±SE. Asterisks indicate mean values significantly lower than positive controls (P < 0·05).