Figure 3.

ACAID induction when recipients are C57BL/6 and T-cell reconstituted B6.lpr/lpr mice. To prepare T-cell reconstituted mice, mice received 4 × 10⁷ naive C57BL/6 T cells intravenously 24 hr prior to use. T cells were isolated from C57BL/6 spleens and lymph nodes using T-cell enrichment columns. PEC from C57BL/6 were prepared as described in legend to Fig. 1 and injected i.v., respectively, into C57BL/6 (left graph) and T-cell reconstituted B6.lpr/lpr (right graph) recipients. Seven days later, all mice were immunized and then ear challenged with OVA as described in Fig. 1. Ear swelling results are presented as mean± SE (n = 5). Asterisks indicate mean values significantly lower than positive controls (P < 0·05).