Figure 3.

Intracellular cytofluorographic analysis of Herpes virus saimiri (HVS)-transformed T lymphocytes from the leucocyte adhesion deficiency (LAD) patient and a healthy control. Dotted vertical lines represent cursor located based on background staining (isotype-matched controls, labelled as ‘negative’). CD3 staining was used as a positive labelling control (labelled as ‘CD3’). CD18 surface expression was previously blocked with pure unlabelled anti-CD18 monoclonal antibody (mAb), as described in the Materials and methods, and then stained with fluorescein isothiocyanate (FITC)-conjugated anti-CD18 mAb with (labelled as ‘CD18’) or without (labelled as ‘CD18 blockage’) surface membrane permeabilization.