Table 2.

The different primers used in this work

CD18 mRNA amplification
Coding	Non-coding	Size (bp)
CD18-1: TATAAGCTTCAGGGCAGACTGGTAGCAAA	CD18–4: CTTAGCAAGCTTGTGCGCCA	1–1007
CD18-5: AACGAATTCGACTACCCATCGGT	CD18–8: CTAGAATTCTCCTGACGGCCTTGTCTTCA	970–2410
HVS genome amplification
Coding	Non-coding	Size (bp)
HVS.1: GAGTTTCCAAAATGTACTAAGCTAAC	HVS.2-ACTAATAAAAAGTTCCACACAACTAAC	89
CD18 genomic DNA amplification
Coding	Non-coding	Localization (size bp)
CD18-1.1: GACATCATGGACCCCACAAGC	CD18–1·2: CTTCAGCACGTGCCTGAAGGC	Exon 4–exon 6 (5400)
I4F-1: CTGAAACTCCAGCCTGCCTGG	I5R-2: TACAAGTGTTGGGGAGCCAGG	Intron 4–intron 5 (572)
Direct sequencing
	Primer	Localization
CD18-2:	AACAAGCTTCTTGACATTCC	Exon 5
CD18-3:	TCCAAGCTTGATGACCTCAG	Exon 5
I5R-2:	TACAAGTGTTGGGGAGCCAGG	Intron 5
I5R-1:	AGATGGAAGGGGAAGTGGAGG	Intron 5
Competitive PCR analysis
Coding	Non-coding	Localization (size bp)
E4-FM: TAMRA-CTTCACAGGGCCGGGGGATCC	E4-R: CTGGTCGCAGGTAAAGCGTCT	Exon 4 (180)
E5-RM: TAMRA-GTCCTGCAGTGCCTGGGCC	I4F-3: GGGGAGAGGGGAGGTCAGGAG	Exon 5–intron 4 (*)
E6-FM: TAMRA-CTTCGGGTCCTTCGTGGACAAG	E6-R: GGGCAGGCGGCGACCTGCATCA	Exon 6 (240)

^*Amplified size in CT-HVS was 331 bp and in LAD-HVS was 162 bp.