Figure 3.

Inhibitory effect of antibodies against SCF and c-kit on collagen lattice gel contraction. Fibroblasts and mast cells were seeded at a 1 : 5 ratio. Antibodies (Ab) against SCF (0·1–10 µg/ml) and c-kit (0·05–1 µg/ml) were added singly or in combination prior to gel casting. As gel contraction was maximally enhanced at 4–6 hr after gel casting, gel diameters were determined at 6 hr and compared with control (fibroblasts only). Inhibition of gel contraction was assessed by the comparison of the alteration of gel diameter between those with and without antibodies.