Figure 2.

Binding and internalization of monoclonal anti-dsDNA antibody (9D7) and mouse IgG2b into Jurkat T cells were compared. Jurkat T cells (3 × 10⁵/ml) were incubated with 9D7 (10 µg/ml) or mouse IgG2b (10 µg/ml) at 4° for 1 hr in binding test and at 37° for 18 hr in internalization test, respectively. After washing with PBS, percentage binding and internalization of anti-dsDNA antibodies were analysed by flow cytometry as described in Methods. The abscissa denotes fluorescence intensity and ordinate denotes cell number.