Figure 7.

Effect of phytohaemagglutinin‐L (PHA‐L), lipopolysaccharide (LPS) and gap junction communication inhibitors on dye transfer between T and B lymphocytes co‐cultured in different combinations. After loaded and non‐loaded cells were allowed to seed for 30 min, PHA‐L (1 µg/ml), LPS (5 µg/ml), 18‐α‐glycyrrhetinic acid (AGA) (150 µm) and GAP27 peptide (300 µg/ml) were added to the different combination groups, respectively, and cells were incubated for 6 hr. Fluorescence was measured by flow cytometry and the percentage of positive green fluorescent cells above the arbitrary marker was analysed in each case. A control group of non‐treated cells was examined in parallel (co‐cultured cells). Results represent the mean ± standard deviation of six experiments for each combination.