Table 1.

Peptides/antibodies used for connexin40 (Cx40) and Cx43 protein expression analysis

Peptide /antibody	Cx	Amino acids	Peptide sequence	Topographical position	Experimental use
GAP18	43	1–16	MGDWSALGKLLDKVQAC	Intracellular N‐terminal tail	ELISA
GAP35	43	44–62	AWGDEQSAFRCNTQQPGC	Extracellular loop 1	FM, FACS
GAP36	43	191–209	KRDPCPHQVDCFLSRPTEK	Extracellular loop 2	FM, FACS
GAP38	40	285–296	NMASQQNTDNLV	C terminus	FM, WB, FACS
GAP27	43	204–214	SRPTEKTIFII	Extracellular loop 2	ELISA
mAbCx43*	43	252–270	GPLSPSKDCGSPKYAYFNG	C terminus	WB

Cx, connexin; ELISA, enzyme‐linked immunosorbent assay; FM, fluorescence microscopy; mAb, monclonal antibody; WB, Western blotting.

^*Commercial mAb (Chemicon).