Table 2.

Reverse transcription–polymerase chain reaction (RT–PCR) analysis for connexin mRNA expression in lymphocyte subpopulations

Name	Sequence	Direction	Expected product size	PCR conditions for pair of primers
HmCx26Up	CCG AAG TTC ATG AAG GGA GAG AT	Sense	390 bp	1 cycle: 94°, 4 min
HmCx26Lo	GGT CTT TTG GAC TTC CCT GAG CA	Antisense		35 cycles: 94°, 1 min; 61°, 1 min; 72°, 1 min 1 cycle: 72°, 8 min
HmCx32Up	CTG CTC TAC CCG GGC TAT GC	Sense	386 bp	1 cycle: 94°, 4 min
HmCx32Lo	CAG GCT GAG CAT CGG TCG CTC TT	Antisense
				35 cycles: 94°, 1 min; 60°, 1 min; 72°, 1 min 1 cycle: 72°, 8 min
RatCx37P1	GAC TGG GGC TTC CTG GAG AAG	Sense	463 bp	1 cycle: 94°, 4 min
RatCx37P2	GCC ACC GAG ATC TTG GCC ATC	Antisense
				35 cycles: 94°, 1 min; 65°, 1 min; 72°, 1 min 1 cycle: 72°, 8 min
RatCx40P1	ATG CAC TGT GCG CAT GCA GGA	Sense	399 bp	1 cycle: 94°, 4 min
RatCx37P2	CAG GTG GTA GTG TTC AGC CAG	Antisense
				35 cycles: 94°, 1 min; 58°, 1 min; 72°, 1 min 1 cycle: 72°, 8 min
HmCx43Up	TAC CAT GCG ACC AGT GGT GCG CT	Sense	294 bp	1 cycle: 94°, 4 min
HmCx43Lo	GAA TTC TGG TTA TCA TCG TCG GGG AA	Antisense
				35 cycles: 94°, 1 min; 60°, 1 min; 72°, 1 min 1 cycle: 72°, 8 min
HmCx45Up	CTA TGC AAT GCG CTG GAA ACA ACA	Sense	815 bp	1 cycle: 94°, 4 min
HmCx45Lo	CCC TGA TTT GCT ACT GGC AGT	Antisense		35 cycles: 94°, 1 min; 55°, 1 min; 72°, 1 min 1 cycle: 72°, 8 min