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. 2000 Apr;99(4):481–488. doi: 10.1046/j.1365-2567.2000.00990.x

Figure 4.

Figure 4

Expression of the laminin α2-chain in the thymus. (a) Western blotting pattern of laminin α2-chain expression in the normal BALB/c thymus. Human merosin (lane 1 as a positive control), the Sephadex G-10 pass-through fraction of thymocytes (lane 2), thymi (lane 3–10, from 54-, 35-, 25-, 21-, 18-, 12-, 7- and 0-day-old mice, respectively), or muscle (lane 11, 54-day-old mice). (b) Western blotting pattern of laminin α2-chain expression in muscle and thymus. Muscle of normal BALB/c (lane 1), mer+/– (lane 2), or mer–/– (lane 3), and thymus of mer+/– (lane 4), or mer–/– (lane 5) mice. (c) Reverse transcription–polymerase chain reaction (RT–PCR) analysis of laminin α2-chain expression in thymic epithelial cell (TEC) lines. RNA from the muscle of normal BALB/c mice (lane 1), TEC line S1Bc (from normal BALB/c mice) (lane 2), TEC line S7wtE1 (from WT mice) (lane 3), S7HoE1 (from mer–/– mice) (lane 4), or Sephadex G-10 pass-through normal thymocytes (lane 5). GAPDH, glyceraldehyde-3-phosphate dehydrogenase; LN, lymph node. (d) Western blotting pattern of laminin α2-chain expression in normal BALB/c mouse spleen. Human merosin (lane 1 as a positive control), spleens (lanes 2–6 from 54-, 40-, 29-, 21- and 12-day-old mice, respectively), thymus (35-day-old mice) (lane 7), or muscle (54-day-old mice) (lane 8).