Figure 6.
Increased viability of normal thymocytes cultured in the presence of merosin or anti-very late activation antigen-6 (VLA-6) monoclonal antibodies (mAbs). Normal BALB/c thymocytes (from 4-week-old mice) were prepared by passing the thymus cells through a Sephadex G-10 column to remove adherent cells. The cells were cultured for 8 hr in serum-free medium with various anti-integrin mAbs or human merosin (protein concentration 10 µg/ml/5 × 105 cells). Immunoglobulin G (IgG) indicates normal rat IgG. 7-amino actinomycin D (7-AAD)-negative cells were counted. The data shown are mean ± SD (of three independent cultures) and are representative of three separate experiments.