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The mAb PPT27 precipitates TCR. 125I‐labelled porcine PBL were lysed either in NP‐40 (a) or digitonin (b) lysis buffer and precipitated with Protein G–Sepharose 4B beads coated with anti‐CD3 mAb PPT3 (lanes 1, 4), mAb PPT27 (lanes 2, 5), or normal mouse serum (lanes 3, 6). Gels (10%) run under non‐reducing (lanes 1–3) or reducing (lanes 4–6) conditions.
