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. 2000 Apr;99(4):504–509. doi: 10.1046/j.1365-2567.2000.00019.x

Figure 4.

Figure 4

The mAb PPT27 recognizes most, but not all, γδ TCR. (a) Immunoprecipitation of PBL subsets. Porcine PBL depleted with anti‐CD4+ anti‐CD8hi (lanes 1–3) or with PPT27 (lanes 4–6) were 125Iodinated and lysed in digitonin lysis buffer before being precipitated with anti‐CD3 (lanes 1, 4), PPT27 (lanes 2, 5) or normal mouse serum (lanes 3, 6). Gel (10%) run under reducing conditions. (b) Sequential immunuoprecipitation. 125Iodinated PBL (lanes 1–4) or CD4– CD8– cells (lanes 5, 6) lysed in digitonin buffer was precleared with anti‐CD3 (lanes 1, 2) or with PPT27 (lanes 3–6) before being precipitated with anti‐CD3 (lanes 1, 3, 5) or with PPT27 (lanes 2, 4, 6). Gel (10%) run under reducing conditions.