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. 2000 Apr;99(4):553–560. doi: 10.1046/j.1365-2567.2000.00985.x

Figure 1.

Figure 1

(a) Phase‐contrast microscopic features of U937 cells stimulated or unstimulated with phorbol 12‐myristate 13‐acetate (PMA). U937 were cultured with or without 10 ng/ml of PMA for 24 hr, and morphological changes were observed by phase‐contrast microscopy. (A) unstimulated U937; (B) PMA‐stimulated U937. Note that U937 were found to be tightly adhered to Petri dishes after stimulation with PMA. Data are representative examples of four similar experiments. (b) Expression of CD14 and CD86 on U937 stimulated or unstimulated with PMA. U937 were cultured with or without 10 ng/ml of PMA for 24 hr, and the expression of CD14 and CD86 on U937 was examined as described in the text. Note that CD14 expression on U937 was induced by PMA, and CD86 expression on U937 was augmented by PMA. Numbers represent the percentage of positive cells. Data are representative examples of four similar experiments.