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. 2000 Apr;99(4):553–560. doi: 10.1046/j.1365-2567.2000.00985.x

Figure 2.

Figure 2

(a) Apoptosis of U937 as determined by flow cytometry. U937 were cultured with or without phorbol 12‐myristate 13‐acetate (PMA) in the presence or absence of pyrrolidinedithiocarbamete (PDTC) for 24 hr, and apoptosis was examined as described in the text. Numbers represent the percentage of hypodiploid DNA‐positive cells. (A) unstimulated U937; (B) PMA‐treated U937; (C) PMA‐treated U937 in the presence of PDTC. Note that apoptosis of U937 was not increased by PMA treatment alone, whereas it was significantly augmented in PMA‐treated U937 in the presence of PDTC. (b) Nuclear NF‐κB activity in U937 as determined by the electrophoretic mobility shift assay (EMSA). U937 were cultured with or without PMA in the presence or absence of PDTC for 6 hr, and nuclear NF‐κB activity in the cells was examined by EMSA, as described in the text. (A) positive control (MT‐2); (B) unstimulated U937; (C) PMA‐treated U937; (D) PMA‐treated U937 in the presence of PDTC. Note that nuclear NF‐κB activity was clearly increased in PMA‐treated U937, whereas it was significantly suppressed by PDTC. Data are representative examples of four similar experiments.