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. 2000 Apr;99(4):600–607. doi: 10.1046/j.1365-2567.2000.00986.x

Figure 2.

Figure 2

Time course of mRNA levels of l-histidine decarboxylase (HDC) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in the leucocytes infiltrating the pouch fluid. (a) Leucocytes infiltrating the pouch fluid were collected, at the time-points indicated, after injection of the antigen solution into the air pouch of the immunized and the non-immunized rats. Total RNA was extracted and reverse transcription–polymerase chain reaction (RT–PCR) was performed as described in the Materials and methods. The mRNA levels were determined in the leucocytes from three individual rats in both groups. (b) The ratio of HDC mRNA density to GAPDH mRNA density is shown. Values represent the mean ± SEM from three individual rats. Statistical significance: ***P < 0·001, **P < 0·01 versus the corresponding non-immunized group. The ratio in the non-immunized rats at 2 hr was set to 1·0.