Figure 3.

Reverse transcription–polymerase chain reaction (RT–PCR) analysis of T-cell receptor (TCR) γ and δ mRNA. RNA was extracted from thymocytes (at embryonic day [E]16.5 and 10 weeks old) and skin (at birth and 4 weeks) of wild-type (WT) and lck-promoter terminal deoxynucleotidyl transferase (lckTdT)^+/+ mice. cDNA was amplified by PCR using a Cγ primer and one of five Vγ (Vγ1-Vγ5) primers (a) or with a Cδ primer and one of seven Vδ (Vδ1-Vδ7) primers (b), and an aliquot was separated by electrophoresis and transferred onto nylon membranes. The blotted membranes were hybridized with a Cγ probe (a) or a Cδ probe (b). Hypoxanthine-guanine phosphoribosyl transferase (HPRT) was used as an internal control for loading equal amounts of cDNA for analysis. NB; newborn. (a) 1, Vγ1; 2, Vγ2; 3, Vγ3; 4, Vγ4; 5, Vγ5. (b) 1, Vδ1; 2, Vδ2; 3, Vδ3; 4, Vδ4; 5, Vδ5; 6, Vδ6; 7, Vδ7.