Table 2.
Summary of flow cytometric analysis of fetal thymus
| E15.5(n) | E16.5(n) | E17.5(n) | E18.5(n) | |
|---|---|---|---|---|
| αβ–γδ+ | ||||
| WT | 2·1±0·9*(6) | 2·9±0·7 (7) | 2·9±0·8 (8) | 1·9±0·2 (5) |
| C57B/6 | 2·0±0·6 (6) | 2·6±0·6 (7) | 2·4±0·6 (7) | 2·7±0·3 (6) |
| 129 | 1·7 (1) | 2·9±0·3 (6) | 2·6*(1) | 1·6±0·4 (7) |
| lckTdT+/+ | 0·7±0·1 (5) | 1·2±0·2 (7) | 0·9±0·2 (9) | 1·0±0·2 (16) |
| Vγ3+Thy–1·2+ | ||||
| WT | 2·0±0·3 (7) | 2·2±0·5 (9) | 1·8±0·4 (6) | 1·0±0·5 (5) |
| C57BL/6 | 1·8±0·4 (8) | 3·0±0·6 (10) | 2·0±0·4 (7) | 0·9±0·2*(6) |
| 129 | 2·0*(1) | 3·7±0·1 (4) | 1·9*(1) | 1·1±0·3*(7) |
| lckTdT+/+ | 0·9±0·2 (5) | 1·0±0·3 (10) | 0·8±0·1 (10) | 0·6±0·1 (12) |
| Vγ3+HSA+ | ||||
| WT | 1·4±0·4*(9) | 2·7±0·5 (8) | 2·0±0·6 (8) | 1·1±0·4*(5) |
| C57BL/6 | 1·7±0·6*(7) | 2·5±0·3 (9) | 1·7±0·4 (7) | 1·0±0·4 (6) |
| 129 | 1·3*(1) | 2·6±0·5 (4) | 2·0*(1) | 1·2±0·3 (7) |
| lckTdT+/+ | 0·7±0·1 (5) | 1·2±0·2 (3) | 1·1±0·1 (12) | 0·5±0·2 (18) |
All values represent mean percentages ± SD. The population of Vγ3+ heat-stable antigen (HSA)+ cells includes Vγ3+HSAhigh cells and Vγ3+HSAlow cells. The percentages in lck-promoter terminal deoxynucleotidyl transferase (lckTdT)+/+ mice were significantly lower than those in the three control groups at P < 0·001 (no asterisk)
*
P < 0·01
**
P < 0·05
***
denotes no significant difference.
Statistical analysis was performed by using one-way analysis of variance (anova) with statview4·5.
WT, wild-type littermates; n, number of embryos analysed.