Table 3.
Summary of Vγ3-Jγ1/Vδ1-Dδ-Jδ2 junctional sequences in fetal thymus
| Number of seq. analysed | In-frame seq.(%) | N+ seq. (%) | Canonical seq. (%) | Canonical seq./in-frame (%) | ||
|---|---|---|---|---|---|---|
| Vγ3-Jγ1 junctional sequences | ||||||
| C57BL/6 | ||||||
| E16.5 | genome | 37 | 14 (38) | 1 (3) | 14 (38) | 14/14 (100) |
| E16.5 | cDNA | 35 | 32 (91) | 1 (3) | 28 (80) | 28/32 (88) |
| lckTdT+/+ | ||||||
| E14.5 | genome | 37 | 14 (38) | 25 (68) | 4 (11) | 4/14 (29) |
| E15.5 | genome | 39 | 17 (44) | 27 (69) | 5 (13) | 5/17 (29) |
| E16.5 | genome | 43 | 24 (56) | 32 (74) | 6 (14) | 6/24 (25) |
| E17.5 | genome | 37 | 19 (51) | 25 (68) | 5 (14) | 5/19 (26) |
| E16.5 | cDNA | 44 | 35 (80) | 20 (45) | 16 (36) | 16/35 (46) |
| E17.5 | cDNA | 45 | 34 (76) | 24 (53) | 17 (38) | 17/34 (50) |
| Vδ1-Dδ-Jδ2 junctional sequences | ||||||
| C57BL/6 | ||||||
| 16.5 | genome | 37 | 33 (89) | 1 (3) | 33 (89) | 33/33 (100) |
| E16.5 | cDNa | 36 | 35 (97) | 1 (3) | 29 (81) | 29/35 (83) |
| lckTdT+/+ | ||||||
| E16.5 | genome | 33 | 15 (45) | 26 (79) | 3 (9) | 3/15 (20) |
| E16.5 | cDNA | 25 | 23 (92) | 17 (68) | 1 (4) | 1/23 (4) |
Junctional sequences (seq.) of genomic DNA and cDNA from embryonic day (E)14.5–17.5 thymuses of control C57BL/6 mice and lck-promoter terminal deoxynucleotidyl transferase (lckTdT)+/+ mice. Two embryos were analysed at each age, and two independent polymerase chain reaction (PCR) amplification reactions were performed for each embryo. Identical sequences detected in products from the same PCR reaction were counted only once, except when they were found multiple times in independent amplifications.