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. 2008 Apr 18;105(17):6302–6307. doi: 10.1073/pnas.0802091105

Fig. 1.

Fig. 1.

Identification of RETRA. (a) Structural formula of RETRA [2-(4,5-dihydro-1,3-thiazol-2-ylthio)-1-(3,4-dihydroxyphenyl)ethanone hydrobromide]. (b) Induction of β-galactosidase reporter in A431/LC5 cells after 14-h incubation with 4 μM RETRA, 0.05% DMSO. The upper control segment corresponds to the treatment with diluted DMSO (0.05%). Staining with X-Gal. (c) Induction of β-galactosidase reporter in cell lines expressing mutant p53 Arg273His (A431, SW480, HT29, SW620), Arg248Trp (SW837), Arg280Lys (MDA-MB-231), and Gly266Glu (MDA-MB-435) and treated at IC50 (4 μM) with RETRA for 14 h (ONPG reaction). (d) Induction of β-galactosidase reporter in the p53-null cell lines MDA041, H1299, Saos-2, and PC3 and in the wild-type p53-expressing cells HeLa, A549, and HEFs, treated as above.