Fig. 3.

Weak RPM1 function in Mt-0 is suppressed posttranscriptionally by tao1–5. (A) Four-week old plants were hand-infiltrated with Pto DC3000(avrB) (Upper) or Pto DC3000(avrRpm1) (Lower) at 10⁵ cfu/ml. Error bars represent the SD among four samples. This experiment is indicative of three independent replicates. Similar results were seen with dip infections (data not shown). (B and C) Western blot analysis showing RPM1-myc accumulation in plant lines with the noted genotypes. Cross-reacting band indicates equivalent amounts of total protein loading. Samples from hybrid (F₁) and parental (P₁) lines are noted above their respective genotypes. These experiments are indicative of four (B) and two (C) independent replicates. The higher than wild-type level of RPM-myc accumulation in the tao1–2 background shown in B was not reproducible.