Figure 3.

Zinc sensitivity of extrasynaptic NMDARs in PCs from adult mice A, single-channel currents activated by 100 μm NMDA (50 μm glycine) before and after the application of 5 or 200 nm Zn²⁺ (P74; −60 mV) in an outside-out patch exhibiting ‘high’ zinc sensitivity. Bar plot shows pooled data from 11 experiments (all mice older than P70) and illustrates the reduction of NMDAR-mediated charge transfer (Q(t)) in the presence of Zn²⁺ (symbols indicate values from individual patches; the number of patches is indicated in brackets). Note that, on average, the sensitivity of NMDARs to Zn²⁺ in these patches is typical of recombinant NR2A-containing NMDARs (as indicated by the dotted lines; see Rachline et al. 2005). B, single-channel currents in a patch exhibiting ‘low’ zinc sensitivity (P77; −60 mV). Bar plots illustrate pooled data from 3 experiments. Dotted lines indicate the level of Zn²⁺ inhibition expected for recombinant NR2B-containing NMDARs (see Rachline et al. 2005).