Figure 2.

Schematic diagram of the UGPase1 gene and derived cleaved amplified polymorphic sequence (dCAPS) marker analysis.

1. The mutation within the UGPase1 gene in the ms-h mutant.
2. dCAPS marker development for detection of the 1-bp substitution at the 3′-splice junction of the 14th intron. The dCAPS marker using a mismatch primer selectively generates a restriction site (SpeI) in the mutant, but not in the wild-type parent. Lowercase letters of sequences indicate the 14th intron and uppercase letters indicate the 15th exon.
3. dCAPS marker genotype of F₂ plants from Hwacheong ms-h mutant × Hwacheong, classified by phenotype. After digestion with restriction enzyme SpeI, a single, 196-bp PCR product was observed in fertile homozygotes (genotype 1), whereas in the male-sterile homozygotes (genotype 3), a shorter, 169-bp PCR product was observed, resulting from the generation of a new SpeI recognition site resulting from the single nucleotide substitution. In fertile heterozygotes (genotype 2), both fragments are observed. Genotype 1, Ms-h/Ms-h; genotype 2, Ms-h/ms-h; genotype 3, ms-h/ms-h.