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. 2008 Apr 1;54(2):190–204. doi: 10.1111/j.1365-313X.2008.03405.x

Figure 7.

Figure 7

Molecular analysis of the UGPase1 gene complemented plants (T0) and T1 plants.
  1. RT-PCR analysis of T0 plants.
  2. Spikelet fertility and the ratio of normal to opaque seed of T1 plants derived from T0 plants (c10 and c13).
  3. RT-PCR analysis of T1 plants derived from T0 plants (c10 and c13). The upper panel shows a gel electrophoresis pattern resulting from RT-PCR using the UGP1-PRT primer set. The lower panel shows gel electrophoresis following RT-PCR of the ubiquitin gene as a control. F, wild-type plant (fertile Hwacheong); M, Hwacheong gms mutant; c2, c3, c6, c9, c10, c12, c13, c16, c22, c24 and c29, complemented plants; V1 and V2, empty vector transformed plants; 10-N1, 10-N2, 10-N3, 10-N4, 10-N5 and 10-N6, T1 plants derived from normal seeds of the c10 transformant; 10-O, T1 plant derived from opaque seeds of the c10 transformant; 13-N3, T1 plant derived from a normal seed of the c13 transformant; 13-O, T1 plant derived from an opaque seed of the c13 transformant; Ubq, ubiquitin.