Table 1.
The PCR-based molecular markers designed for fine mapping
| CAPS | Forward primer (5′→3′) | Reverse primer (5′→3′) | Fragment size amplified in japonica (bp) | Restriction enzyme | Originated clone |
|---|---|---|---|---|---|
| 869a | CTTCCCCGAGGTAGGTGCTA | CAGGCACATCAACAATTCCA | 1296 | RsaI | AP006548 |
| 869c | TCCAGCAGAGTCTCCATCAA | CACAGTCATCACATGCATCATT | 1377 | AluI, MspI | AP006548 |
| RG451 | TCCATAAGATCGTTCATCTGG | GTGTAAACCCTGGATGTGATG | 550 | MnlI | AP005862 |
| 18420b | TTTTGGTCGTGACCGTGTAA | AGGCTCATATCAACGCGAAA | 1311 | AluI, MnlI, Tru9I, | AP006149 |
| 2505a | AAAAATCTTGGCACCAGAGG | GAATTTTGATGTGGGAGCTG | 1582 | XbaI, DraI, Sau3AI | AP006548 |
| STS | Forward primer (5′→3′) | Reverse primer (5′→3′) | Fragment size amplified in japonica (bp) | Originated clone |
|---|---|---|---|---|
| SSR592 | ACATCATGGGCTTTCCAAAC | GCTATCCGATCGATACCTTCC | 269 | AP005742 |
| 5564m | CACTTTGGTTAGGCCGACTC | GCGTAAGACCTCCCTCCAAT | 165 | AP005564 |
| 5564t | CAGGTGACCAGGTGGAATTT | TGCCTACTTTGGGTTTGTTTG | 422 | AP005564 |
| 5564p | CGGATCAGCTAAGAGCGATT | ACCACGCGAGGTATGAGC | 156 | AP005564 |
| 5564v | TCTCCATGACCAACCTATTGC | CAAGGGAGAGTTTCCTCACG | 160 | AP005564 |
| 5564s | CTCTTGCCGTGCTATGTGAA | TCAAACTCCAAAACCCAAGC | 396 | AP005564 |
| 5564d | CCTGCCATCTCTTCAAGCAT | TCAAGTTCACACAGCCAAGT | 292 | AP005564 |
| 7596c | CAAAGCGGACAGAAAACGAT | TCTGGTTTTTAGCTATGCCGTA | 136 | AC137596 |
| 7596d | CGGCTTCTTTCCTCTTTCG | GGAGTATGAGGAGGGGAAGG | 178 | AC137596 |
| 7596e | TCGCTACTTTTACCGCATCC | CAAAACCAGTGGGCTACACC | 221 | AC137596 |
| 7596f | GAACTTTAGAAAAGGTAAGGCTTCT | CAGTTTGATTGCACCATTGC | 188 | AC137596 |
| 7596b | GATGACGCCCAACAATCTCT | GGACTATAGGCCGTTCCTTG | 143 | AC137596 |
| 7596a | TCTGAGTGGTTGGTTTGTCG | GGGTGTACTGTGGGATTTCG | 178 | AC137596 |
| 7593a | AAGAACATGTACCCTACGAACACA | TTTTTCTTCCTCACCAGAACAA | 279 | AC137593 |
| 2462d | TTCTTTTTCATGCCCCACTC | GATCCGGACAGGTTCGTTTA | 1526 | AC137593 |