Figure 5.
Constitutively active Gαi2 reduces stimulus-evoked vesicular release from Schaffer collateral presynaptic terminals. (A) Typical two-photon image of Schaffer collateral presynaptic puncta, samples marked with white squares, labeled with FM1-43 in stratum radiatum of a hippocampal slice; 800 nm excitation, >600 nm emission filtering. (B) Time-course of stimulus-induced reductions in FM1-43 bouton fluorescence evoked by a 10 Hz/2 min train of stimulation (solid bar) applied to Schaffer collateral axons in slices from wild-type (◦) and Gαi2-expressing double-transgenic (●) mice. Each point is mean ± SEM of 20–24 puncta per slice from four wild-type and five double-transgenic mice.