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. 2008 Apr;15(4):261–270. doi: 10.1101/lm.810208

Figure 7.

Figure 7.

Elevating the concentration of cyclic GMP elicits transient depression of Schaffer collateral synaptic transmission in control mice, but LTD in mice expressing constitutively active Gαi2. (A) Time course comparing the effect of bath application of zaprinast alone (ZAP, 20 μM, hatched bar; n = 7) on Schaffer collateral-CA1 evoked EPSCs in slices from single-transgenic control mice (◦), to chemical LTD induced by combining zaprinast with the selective PKA inhibitor H-89 (H-89, 10 μM, open bar; ●; n = 6). Each point is mean ± SEM of n slices. (B) Time course of the effect of bath application of zaprinast (ZAP, 20 μM, hatched bar) on Schaffer collateral-CA1 evoked EPSPs in slices from Gαi2-expressing double-transgenic (●; n = 9) versus pooled single-transgenic slices (◦; n = 7). Each point is mean ± SEM of n slices. (C) Time course of the effect of bath application of zaprinast (ZAP, 20 μM, hatched bar) on Schaffer collateral-CA1 evoked EPSCs in slices from Gαi2 double-transgenic mice taken off doxycycline 5 d earlier (off Dox; ●; n = 9) versus slices from double-transgenic mice where expression was blocked by doxycycline (on Dox; ◦; n = 4) Each point is mean ± SEM of n slices.