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. 2008 May;14(5):914–927. doi: 10.1261/rna.829208

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FIGURE 8. — ATPase activity of UPF1 is required for HIV-1 up-regulation. Cells were (lane 1) mock transfected or co-transfected with HIV-1 proviral DNA (pNL4-3, HIV-1) with Flag, UPF1^WT, TDN (R844C), UPF1^RRAA, and UPF1^DE. At 30 h post-transfection, cells were harvested for Western blot analysis for UPF1 expressed in trans using an anti-Flag epitope tag, UPF2 (as loading control), Gag, and GAPDH (as loading control). The relative levels of pr55^Gag are related to the signal obtained in Flag and HIV-1-expressing cells, set to 1. The histogram shows the averages (±SD).