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miR-148 reduces endogenous Dnmt3b1 mRNA and protein level. (A) qRT-PCR of either miR-Vec-148 or control (Ctrl.) transfected HeLa cells. Specific primers were used to detect Dnmt3b1 and Dnmt3b3 as shown schematically. Error bars represent standard deviation, n = 4 and (***) P < 0.001. (B) 2102EP cells were electroporated with the indicated constructs and subjected to immunoblot analysis for DNMT3b and TUBULIN.
