Figure 1.
Comparison of FACS profiles of marrow cells from NOD/SCID mice transplanted 6 and 8 weeks previously with 300 CD34+CD38− cells (A and B) (i.e., at limiting dilution) and 3 × 103 CD34+CD38− cells (C) from the same original CB sample. In A the cells were stained with an irrelevant (control) isotype-matched mouse IgG. In B and C, the cells were stained with anti-human CD34–CY5 (cyanine-5-succinimidyl) and anti-human CD19–PE. Evidence of human cells of all three phenotypes examined in these analyses is seen in both mice (B and C).