Figure 4.

RT-PCR analysis of SDF-1 mRNA content of primary and transformed CD8⁺ effector cells. (A) Autoradiograph of a Southern blot of RT-PCR products from a dilution series of an in vitro SDF-1 transcript. The blot was probed with a ³²P-labeled SDF-1 riboprobe. (B) Ethidium bromide-stained agarose gel of RT-PCR products of total RNA extracted from either HVS-transformed CD8⁺ cells, primary CD8⁺ cells from HIV⁺ and HIV⁻ subjects, unfractionated PBMC from a pool of HIV⁻ donors, and control tissues as noted. OV, ovarian tumor cells; BR, breast tumor cells. (C) Autoradiograph of a Southern blot of the gel in B probed with a ³²P-labeled SDF-1 riboprobe. (D) Ethidium bromide-stained agarose gel bearing actin RT-PCR products amplified from the same RNA preparations used for the amplifications in B.